› 6rgt

X-ray diffraction
1.6Å resolution

Crystal structure of the cofactor-free Aspergillus flavus urate oxidase T57A variant anaerobically complexed with 9-methyl uric acid

Released:
DOI: 10.2210/pdb6rgt/pdb
PDB entry 6rgt coloured by chain, front view.
PDB entry 6rgt coloured by chain, side view.
PDB entry 6rgt coloured by chain, top view.
Source organism: Aspergillus flavus
Entry authors: Bui S, Lowden SRJ, Steiner RA

Function and Biology Details

Reaction catalysed: 
Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uricase Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.26 KDa
Source organism: Aspergillus flavus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00511 (Residues: 1-302; Coverage: 100%)
Gene names: Uox, uaZ
Sequence domains: Uricase

Ligands and Environments

3 bound ligands:
Ligand MUA 1 x MUA
Ligand MPD 1 x MPD
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION NOVA
Spacegroup: I222
Unit cell:
a: 79.291Å b: 95.116Å c: 104.503Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.189
Expression system: Escherichia coli BL21(DE3)

Quick links