› 6r2j

X-ray diffraction
1.44Å resolution

Crystal Structure of Pseudomonas stutzeri endoglucanase Cel5A in complex with cellobiose

Released:
DOI: 10.2210/pdb6r2j/pdb
PDB entry 6r2j coloured by chain, front view.
PDB entry 6r2j coloured by chain, side view.
PDB entry 6r2j coloured by chain, top view.
Source organism: Pseudomonas stutzeri A1501
Primary publication:
Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.
Dutoit R, Delsaute M, Collet L, Vander Wauven C, Van Elder D, Berlemont R, Richel A, Galleni M, Bauvois C
Acta Crystallogr D Struct Biol 75 605-615 (2019)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107450 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glycoside hydrolase family 5 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 330 amino acids
Theoretical weight: 37.22 KDa
Source organism: Pseudomonas stutzeri A1501
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4VME5 (Residues: 31-360; Coverage: 100%)
Gene name: PST_2494
Sequence domains: Cellulase (glycosyl hydrolase family 5)

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
1 bound ligand:
Ligand TRS 4 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P21
Unit cell:
a: 70.25Å b: 82.82Å c: 104.68Å
α: 90° β: 91.97° γ: 90°
R-values:
R R work R free
0.181 0.179 0.224
Expression system: Escherichia coli BL21(DE3)

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Citations

5 citation in other articles

Bioaugmentation potential of inoculum derived from anaerobic digestion feedstock for enhanced methane production using water hyacinth.
Obi et al. (2023)
4 more