› 6pmh

X-ray diffraction
2.3Å resolution

Structure of Epimerase Mth375 from the thermophilic pseudomurein-containing methanogen Methanothermobacter thermautotrophicus

Released:
DOI: 10.2210/pdb6pmh/pdb
PDB entry 6pmh coloured by chain, front view.
PDB entry 6pmh coloured by chain, side view.
PDB entry 6pmh coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Entry authors: Carbone V, Schofield LR, Sutherland-Smith AJ, Ronimus RS

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127839 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent epimerase/dehydratase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 368 amino acids
Theoretical weight: 41.97 KDa
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Expression system: Escherichia coli
UniProt:
  • Canonical: O26475 (Residues: 1-332; Coverage: 100%)
Gene name: MTH_375
Sequence domains: NAD dependent epimerase/dehydratase family

Ligands and Environments

5 bound ligands:
Ligand PO4 1 x PO4
Ligand GOL 2 x GOL
Ligand CL 2 x CL
Ligand UDP 1 x UDP
Ligand AMP 1 x AMP
1 modified residue:
Ligand CME 1 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: R32
Unit cell:
a: 113.815Å b: 113.815Å c: 239.873Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.149 0.147 0.178
Expression system: Escherichia coli

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