› 6omz

X-ray diffraction
1.85Å resolution

Crystal Structure of Dihydropteroate synthase from Mycobacterium smegmatis with bound 6-hydroxymethylpterin-monophosphate

Released:
DOI: 10.2210/pdb6omz/pdb
PDB entry 6omz coloured by chain, front view.
PDB entry 6omz coloured by chain, side view.
PDB entry 6omz coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MKD8
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydropteroate synthase Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 30.52 KDa
Source organism: Mycolicibacterium smegmatis MKD8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A2U9PYL8 (Residues: 1-285; Coverage: 100%)
Gene name: D806_059810
Sequence domains: Pterin binding enzyme

Ligands and Environments

4 bound ligands:
Ligand CIT 2 x CIT
Ligand DMS 1 x DMS
Ligand PMM 1 x PMM
Ligand EDO 8 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2221
Unit cell:
a: 47.72Å b: 96.41Å c: 135.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.151 0.186
Expression system: Escherichia coli BL21(DE3)

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