› 6o23

X-ray diffraction
2Å resolution

Crystal structure of 2243 Fab in complex with circumsporozoite protein NANP5

Released:
DOI: 10.2210/pdb6o23/pdb
PDB entry 6o23 coloured by chain, front view.
PDB entry 6o23 coloured by chain, side view.
PDB entry 6o23 coloured by chain, top view.
Source organisms:
Primary publication:
Evolution of protective human antibodies against Plasmodium falciparum circumsporozoite protein repeat motifs.
Murugan R, Scally SW, Costa G, Mustafa G, Thai E, Decker T, Bosch A, Prieto K, Levashina EA, Julien JP, Wardemann H
Nat Med 26 1135-1145 (2020)
PMID: 32451496

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210765 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
2243 Fab heavy chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 227 amino acids
Theoretical weight: 24.16 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
2243 kappa light chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 213 amino acids
Theoretical weight: 23.33 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Circumsporozoite protein Chain: E
Molecule details ›
Chain: E
Length: 20 amino acids
Theoretical weight: 2 KDa
Source organism: Plasmodium falciparum
Expression system: Not provided
UniProt:
  • Canonical: P19597 (Residues: 253-272; Coverage: 5%)
Gene name: CSP

Ligands and Environments

3 bound ligands:
Ligand EDO 13 x EDO
Ligand SO4 10 x SO4
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 79.148Å b: 67.9Å c: 100.357Å
α: 90° β: 106.2° γ: 90°
R-values:
R R work R free
0.187 0.186 0.224
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

3 review citations

Host-parasite interactions during Plasmodium infection: Implications for immunotherapies.
Chandley et al. (2022)
2 more

1 mention without citation

Structural basis of epitope selectivity and potent protection from malaria by PfCSP antibody L9.
Martin et al. (2023)