6nzd

Electron Microscopy
3.6Å resolution

Cryo-EM Structure of the Lysosomal Folliculin Complex (FLCN-FNIP2-RagA-RagC-Ragulator)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-128944 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
Ragulator complex protein LAMTOR1 Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.33 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6IAA8 (Residues: 5-161; Coverage: 98%)
Gene names: C11orf59, LAMTOR1, PDRO, PP7157
Sequence domains: Late endosomal/lysosomal adaptor and MAPK and MTOR activator
Ragulator complex protein LAMTOR2 Chain: B
Molecule details ›
Chain: B
Length: 127 amino acids
Theoretical weight: 13.65 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9Y2Q5 (Residues: 1-125; Coverage: 100%)
Gene names: HSPC003, LAMTOR2, MAPBPIP, ROBLD3
Sequence domains: Roadblock/LC7 domain
Ragulator complex protein LAMTOR3 Chain: C
Molecule details ›
Chain: C
Length: 124 amino acids
Theoretical weight: 13.64 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UHA4 (Residues: 1-124; Coverage: 100%)
Gene names: LAMTOR3, MAP2K1IP1, MAPKSP1, PRO2783
Sequence domains: Mitogen-activated protein kinase kinase 1 interacting
Ragulator complex protein LAMTOR4 Chain: D
Molecule details ›
Chain: D
Length: 99 amino acids
Theoretical weight: 10.75 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q0VGL1 (Residues: 1-99; Coverage: 100%)
Gene names: C7orf59, LAMTOR4
Ragulator complex protein LAMTOR5 Chain: E
Molecule details ›
Chain: E
Length: 173 amino acids
Theoretical weight: 18.18 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O43504 (Residues: 1-91; Coverage: 100%)
Gene names: HBXIP, LAMTOR5, XIP
Sequence domains: Ragulator complex protein LAMTOR5
Ras-related GTP-binding protein A Chain: F
Molecule details ›
Chain: F
Length: 313 amino acids
Theoretical weight: 36.62 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q7L523 (Residues: 1-313; Coverage: 100%)
Gene name: RRAGA
Sequence domains: Gtr1/RagA G protein conserved region
Ras-related GTP-binding protein C Chain: G
Molecule details ›
Chain: G
Length: 404 amino acids
Theoretical weight: 44.76 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9HB90 (Residues: 1-399; Coverage: 100%)
Gene name: RRAGC
Sequence domains: Gtr1/RagA G protein conserved region
Folliculin Chain: H
Molecule details ›
Chain: H
Length: 622 amino acids
Theoretical weight: 69.14 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8NFG4 (Residues: 1-579; Coverage: 100%)
Gene names: BHD, FLCN
Sequence domains:
Folliculin-interacting protein 2 Chain: I
Molecule details ›
Chain: I
Length: 1117 amino acids
Theoretical weight: 122.48 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9P278 (Residues: 1-1114; Coverage: 100%)
Gene names: FNIP2, FNIPL, KIAA1450, MAPO1
Sequence domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-0554
Expression systems:
  • Spodoptera frugiperda
  • Homo sapiens