› 6ns3

X-ray diffraction
2.84Å resolution

Crystal structure of fungal lipoxygenase from Fusarium graminearum. I222 crystal form.

Released:
DOI: 10.2210/pdb6ns3/pdb
PDB entry 6ns3 coloured by chain, front view.
PDB entry 6ns3 coloured by chain, side view.
PDB entry 6ns3 coloured by chain, top view.
Source organism: Fusarium graminearum PH-1
Primary publication:
An ensemble of lipoxygenase structures reveals novel conformations of the Fe coordination sphere.
Pakhomova S, Boeglin WE, Neau DB, Bartlett SG, Brash AR, Newcomer ME
Protein Sci 28 920-927 (2019)
PMID: 30861228

Function and Biology Details

Reaction catalysed: 
Linoleate + O(2) = (9Z,12Z)-(11S)-11-hydroperoxyoctadeca-9,12-dienoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125022 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Manganese lipoxygenase Chain: A
Molecule details ›
Chain: A
Length: 769 amino acids
Theoretical weight: 86.41 KDa
Source organism: Fusarium graminearum PH-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I1REW2 (Residues: 1-745; Coverage: 100%)
Gene names: FG02216.1, FGRAMPH1_01T05341
Sequence domains: Lipoxygenase

Ligands and Environments

1 bound ligand:
Ligand FE2 1 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CAMD BEAMLINE GCPCC
Spacegroup: I222
Unit cell:
a: 92.931Å b: 95.537Å c: 186.249Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.234 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Molecular, in silico and expression studies on lipoxygenases (LOXs) in potato (Solanum tuberosum L.).
Kaur et al. (2023)

1 mention without citation

An ensemble of lipoxygenase structures reveals novel conformations of the Fe coordination sphere.
Pakhomova et al. (2019)