› 6mxt

X-ray diffraction
2.96Å resolution

Crystal structure of human beta2 adrenergic receptor bound to salmeterol and Nb71

Released:
DOI: 10.2210/pdb6mxt/pdb
PDB entry 6mxt coloured by chain, front view.
PDB entry 6mxt coloured by chain, side view.
PDB entry 6mxt coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into binding specificity, efficacy and bias of a β2AR partial agonist.
Masureel M, Zou Y, Picard LP, van der Westhuizen E, Mahoney JP, Rodrigues JPGLM, Mildorf TJ, Dror RO, Shaw DE, Bouvier M, Pardon E, Steyaert J, Sunahara RK, Weis WI, Zhang C, Kobilka BK
Nat Chem Biol 14 1059-1066 (2018)
PMID: 30327561

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231679 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-2 adrenergic receptor; Endolysin Chain: A
Molecule details ›
Chain: A
Length: 472 amino acids
Theoretical weight: 53.53 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00720 (Residues: 2-161; Coverage: 98%)
  • Canonical: P07550 (Residues: 29-365; Coverage: 75%)
Gene names: ADRB2, ADRB2R, B2AR, E
Sequence domains:
nanobody Nb71 Chain: N
Molecule details ›
Chain: N
Length: 122 amino acids
Theoretical weight: 13.47 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

7 bound ligands:
Ligand K5Y 1 x K5Y
Ligand MG 1 x MG
Ligand NI 3 x NI
Ligand OLC 1 x OLC
Ligand HTO 1 x HTO
Ligand OLA 1 x OLA
Ligand P33 1 x P33
1 modified residue:
Ligand YCM 2 x YCM

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 178.682Å b: 52.589Å c: 125.917Å
α: 90° β: 123.859° γ: 90°
R-values:
R R work R free
0.207 0.205 0.245
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

16 review citations

Conformational Basis of G Protein-Coupled Receptor Signaling Versatility.
Wingler et al. (2020)
15 more

18 mentions without citation

G Protein-Coupled Receptors in Asthma Therapy: Pharmacology and Drug Action.
Wendell et al. (2020)
17 more