› 6mri

X-ray diffraction
2.62Å resolution

E. coli cysteine desulfurase SufS E250A with a cysteine persulfide intermediate

Released:
DOI: 10.2210/pdb6mri/pdb
PDB entry 6mri coloured by chain, front view.
PDB entry 6mri coloured by chain, side view.
PDB entry 6mri coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural Evidence for Dimer-Interface-Driven Regulation of the Type II Cysteine Desulfurase, SufS.
Dunkle JA, Bruno MR, Outten FW, Frantom PA
Biochemistry 58 687-696 (2019)
PMID: 30571100

Function and Biology Details

Reactions catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160167 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase Chain: A
Molecule details ›
Chain: A
Length: 420 amino acids
Theoretical weight: 45.84 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P77444 (Residues: 1-406; Coverage: 100%)
Gene names: JW1670, b1680, csdB, sufS, ynhB
Sequence domains: Aminotransferase class-V

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
No bound ligands
1 modified residue:
Ligand CSS 1 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43212
Unit cell:
a: 125.73Å b: 125.73Å c: 138.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Fe-S cluster biogenesis by the bacterial Suf pathway.
Blahut et al. (2020)
1 more

2 mentions without citation

Structural Evidence for Dimer-Interface-Driven Regulation of the Type II Cysteine Desulfurase, SufS.
Dunkle et al. (2019)
1 more