› 6m9u

X-ray diffraction
2.2Å resolution

Structure of the apo-form of 20beta-Hydroxysteroid Dehydrogenase from Bifidobacterium adolescentis strain L2-32

Released:
DOI: 10.2210/pdb6m9u/pdb
PDB entry 6m9u coloured by chain, front view.
PDB entry 6m9u coloured by chain, side view.
PDB entry 6m9u coloured by chain, top view.
Source organism: Bifidobacterium adolescentis L2-32
Primary publication:
Structural and biochemical characterization of 20β-hydroxysteroid dehydrogenase from Bifidobacterium adolescentis strain L2-32.
Doden HL, Pollet RM, Mythen SM, Wawrzak Z, Devendran S, Cann I, Koropatkin NM, Ridlon JM
J Biol Chem 294 12040-12053 (2019)
PMID: 31209107

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108200 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxidoreductase, short chain dehydrogenase/reductase family protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 283 amino acids
Theoretical weight: 30.52 KDa
Source organism: Bifidobacterium adolescentis L2-32
Expression system: Escherichia coli
UniProt:
  • Canonical: A7A7R9 (Residues: 12-294; Coverage: 96%)
Gene name: BIFADO_01909
Sequence domains: Enoyl-(Acyl carrier protein) reductase

Ligands and Environments

8 bound ligands:
Ligand IPA 3 x IPA
Ligand MES 1 x MES
Ligand EDO 19 x EDO
Ligand PGE 2 x PGE
Ligand PEG 5 x PEG
Ligand PG4 1 x PG4
Ligand CL 1 x CL
Ligand IMD 2 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: I422
Unit cell:
a: 168.371Å b: 168.371Å c: 126.961Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.231
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Endobolome, a New Concept for Determining the Influence of Microbiota Disrupting Chemicals (MDC) in Relation to Specific Endocrine Pathogenesis.
Aguilera et al. (2020)
2 more

2 mentions without citation

Gut feelings about bacterial steroid-17,20-desmolase.
Ly et al. (2021)
1 more