› 6m94

X-ray diffraction
2.7Å resolution

Monophosphorylated pSer33 b-Catenin peptide bound to b-TrCP/Skp1 Complex

Released:
DOI: 10.2210/pdb6m94/pdb
PDB entry 6m94 coloured by chain, front view.
PDB entry 6m94 coloured by chain, side view.
PDB entry 6m94 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Prospective discovery of small molecule enhancers of an E3 ligase-substrate interaction.
Simonetta KR, Taygerly J, Boyle K, Basham SE, Padovani C, Lou Y, Cummins TJ, Yung SL, von Soly SK, Kayser F, Kuriyan J, Rape M, Cardozo M, Gallop MA, Bence NF, Barsanti PA, Saha A
Nat Commun 10 1402 (2019)
PMID: 30926793

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-152833 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
F-box/WD repeat-containing protein 1A Chain: A
Molecule details ›
Chain: A
Length: 432 amino acids
Theoretical weight: 49.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y297 (Residues: 175-605; Coverage: 71%)
Gene names: BTRC, BTRCP, FBW1A, FBXW1A
Sequence domains:
Structure domains:
S-phase kinase-associated protein 1 Chain: B
Molecule details ›
Chain: B
Length: 144 amino acids
Theoretical weight: 16.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63208 (Residues: 2-163; Coverage: 88%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains:
Catenin beta-1 Chain: C
Molecule details ›
Chain: C
Length: 33 amino acids
Theoretical weight: 3.54 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P35222 (Residues: 17-48; Coverage: 4%)
Gene names: CTNNB, CTNNB1, OK/SW-cl.35, PRO2286

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand DMS 1 x DMS
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P31
Unit cell:
a: 82.37Å b: 82.37Å c: 112.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.203 0.238
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

36 review citations

Wnt signaling in breast cancer: biological mechanisms, challenges and opportunities.
Xu et al. (2020)
35 more