› 6ljd

X-ray diffraction
2.15Å resolution

Crystal structure of fragmin F2-F3 domains (calcium condition)

Released:
DOI: 10.2210/pdb6ljd/pdb
PDB entry 6ljd coloured by chain, front view.
PDB entry 6ljd coloured by chain, side view.
PDB entry 6ljd coloured by chain, top view.
Source organism: Physarum polycephalum
Primary publication:
Novel inter-domain Ca2+-binding site in the gelsolin superfamily protein fragmin.
Takeda S, Fujiwara I, Sugimoto Y, Oda T, Narita A, Maéda Y
J Muscle Res Cell Motil 41 153-162 (2020)
PMID: 31863323

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188196 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gelsolin-like domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 212 amino acids
Theoretical weight: 23.1 KDa
Source organism: Physarum polycephalum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q94707 (Residues: 162-371; Coverage: 57%)
Sequence domains: Gelsolin repeat

Ligands and Environments

5 bound ligands:
Ligand CA 3 x CA
Ligand SO4 1 x SO4
Ligand GOL 1 x GOL
Ligand CL 1 x CL
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AichiSR BEAMLINE BL2S1
Spacegroup: P61
Unit cell:
a: 115.83Å b: 115.83Å c: 42.11Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.193 0.219
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Susceptibility of cytoskeletal-associated proteins for tumor progression.
Ayanlaja et al. (2021)
1 more