› 6laz

X-ray diffraction
2.76Å resolution

the wildtype SAM-VI riboswitch bound to a N-mustard SAM analog M1

Released:
DOI: 10.2210/pdb6laz/pdb
PDB entry 6laz coloured by chain, front view.
PDB entry 6laz coloured by chain, side view.
PDB entry 6laz coloured by chain, top view.
Source organisms:
Primary publication:
SAM-VI riboswitch structure and signature for ligand discrimination.
Sun A, Gasser C, Li F, Chen H, Mair S, Krasheninina O, Micura R, Ren A
Nat Commun 10 5728 (2019)
PMID: 31844059

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-140512 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
U1 small nuclear ribonucleoprotein A Chains: C, D, E
Molecule details ›
Chains: C, D, E
Length: 93 amino acids
Theoretical weight: 10.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09012 (Residues: 6-96; Coverage: 32%)
Gene name: SNRPA
Sequence domains: RNA recognition motif
RNA (55-MER) Chains: A, B
Molecule details ›
Chains: A, B
Length: 55 nucleotides
Theoretical weight: 17.9 KDa
Source organism: Bifidobacterium angulatum
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand E7X 2 x E7X
1 modified residue:
Ligand GTP 2 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 56.39Å b: 85.348Å c: 93.165Å
α: 90° β: 105.46° γ: 90°
R-values:
R R work R free
0.19 0.188 0.223
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Covalent labeling of nucleic acids.
Klöcker et al. (2020)
4 more