› 6kqy

X-ray diffraction
3.3Å resolution

Crystal structure of human leucyl-tRNA synthetase, Leucine-bound form

Released:
DOI: 10.2210/pdb6kqy/pdb
PDB entry 6kqy coloured by chain, front view.
PDB entry 6kqy coloured by chain, side view.
PDB entry 6kqy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Leucine-sensing mechanism of leucyl-tRNA synthetase 1 for mTORC1 activation.
Kim S, Yoon I, Son J, Park J, Kim K, Lee JH, Park SY, Kang BS, Han JM, Hwang KY, Kim S
Cell Rep 35 109031 (2021)
PMID: 33910001

Function and Biology Details

Reaction catalysed: 
ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192839 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine--tRNA ligase, cytoplasmic Chain: A
Molecule details ›
Chain: A
Length: 1188 amino acids
Theoretical weight: 136.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9P2J5 (Residues: 1-1176; Coverage: 100%)
Gene names: KIAA1352, LARS, LARS1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand LEU 2 x LEU
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P6522
Unit cell:
a: 123.744Å b: 123.744Å c: 536.44Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.252 0.251 0.276
Expression system: Escherichia coli

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Citations

4 review citations

Personalized Medicine in Mitochondrial Health and Disease: Molecular Basis of Therapeutic Approaches Based on Nutritional Supplements and Their Analogs.
Tragni et al. (2022)
3 more

2 mentions without citation

O-GlcNAc modification of leucyl-tRNA synthetase 1 integrates leucine and glucose availability to regulate mTORC1 and the metabolic fate of leucine.
Kim et al. (2022)
1 more