› 6jir

X-ray diffraction
1.95Å resolution

Crystal structure of C. crescentus beta sliding clamp with PEG bound to putative beta-motif tethering region

Released:
DOI: 10.2210/pdb6jir/pdb
PDB entry 6jir coloured by chain, front view.
PDB entry 6jir coloured by chain, side view.
PDB entry 6jir coloured by chain, top view.
Source organism: Caulobacter vibrioides CB15
Primary publication:
Caulobacter crescentus β sliding clamp employs a noncanonical regulatory model of DNA replication.
Jiang X, Zhang L, An J, Wang M, Teng M, Guo Q, Li X
FEBS J 287 2292-2311 (2020)
PMID: 31725950

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143193 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta sliding clamp Chains: A, B
Molecule details ›
Chains: A, B
Length: 392 amino acids
Theoretical weight: 42.56 KDa
Source organism: Caulobacter vibrioides CB15
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0CAU5 (Residues: 1-372; Coverage: 100%)
Gene names: CC_0156, dnaN
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand GOL 1 x GOL
Ligand EDO 5 x EDO
Ligand P6G 1 x P6G
Ligand PEG 1 x PEG
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 112.724Å b: 123.527Å c: 85.975Å
α: 90° β: 120.01° γ: 90°
R-values:
R R work R free
0.171 0.171 0.219
Expression system: Escherichia coli BL21

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Citations

3 citation in other articles

Spatial coupling between DNA replication and mismatch repair in Caulobacter crescentus.
Chai et al. (2021)
2 more