› 6im3

X-ray diffraction
2Å resolution

Crystal structure of a highly thermostable carbonic anhydrase from Persephonella marina EX-H1

Released:
DOI: 10.2210/pdb6im3/pdb
PDB entry 6im3 coloured by chain, front view.
PDB entry 6im3 coloured by chain, side view.
PDB entry 6im3 coloured by chain, top view.
Source organism: Persephonella marina EX-H1
Primary publication:
Crystal Structure of a Highly Thermostable α-Carbonic Anhydrase from Persephonella marina EX-H1.
Kim S, Sung J, Yeon J, Choi SH, Jin MS
Mol Cells 42 460-469 (2019)
PMID: 31250619

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111035 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 243 amino acids
Theoretical weight: 27.72 KDa
Source organism: Persephonella marina EX-H1
Expression system: Escherichia coli
UniProt:
  • Canonical: C0QRB5 (Residues: 1-243; Coverage: 100%)
Gene name: PERMA_1443
Sequence domains: Eukaryotic-type carbonic anhydrase

Ligands and Environments

4 bound ligands:
Ligand ZN 6 x ZN
Ligand AZM 6 x AZM
Ligand CA 16 x CA
Ligand PG4 2 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21212
Unit cell:
a: 119.04Å b: 120.811Å c: 123.915Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.24
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Comparison of Carbonic Anhydrases for CO2 Sequestration.
Steger et al. (2022)
3 more

2 mentions without citation

Crystal Structure of a Highly Thermostable α-Carbonic Anhydrase from Persephonella marina EX-H1.
Kim et al. (2019)
1 more