› 6gvn

X-ray diffraction
2.69Å resolution

Tubulin:TM-3 DARPin complex

Released:
DOI: 10.2210/pdb6gvn/pdb
PDB entry 6gvn coloured by chain, front view.
PDB entry 6gvn coloured by chain, side view.
PDB entry 6gvn coloured by chain, top view.
Source organisms:
Primary publication:
Insight into microtubule nucleation from tubulin-capping proteins.
Campanacci V, Urvoas A, Cantos-Fernandes S, Aumont-Nicaise M, Arteni AA, Velours C, Valerio-Lepiniec M, Dreier B, Plückthun A, Pilon A, Poüs C, Minard P, Gigant B
Proc Natl Acad Sci U S A 116 9859-9864 (2019)
PMID: 31036638

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-218891 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWZ0 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Structure domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWY9 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Structure domains:
TM-3 DARPIN Chain: F
Molecule details ›
Chain: F
Length: 169 amino acids
Theoretical weight: 18.05 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

5 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
Ligand SO4 1 x SO4
Ligand MES 1 x MES
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P21
Unit cell:
a: 73.1Å b: 84.26Å c: 81.98Å
α: 90° β: 99° γ: 90°
R-values:
R R work R free
0.194 0.193 0.216
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Mechanism of Tubulin Assembly into Microtubules: Insights from Structural Studies.
Knossow et al. (2020)
1 more

1 mention without citation

Insight into microtubule nucleation from tubulin-capping proteins.
Campanacci et al. (2019)