› 6gna

X-ray diffraction
1.3Å resolution

Crystal structure of a Ferredoxin-Flavin Thioredoxin Reductase from Clostridium acetobutylicum at 1.3 A resolution

Released:
DOI: 10.2210/pdb6gna/pdb
PDB entry 6gna coloured by chain, front view.
PDB entry 6gna coloured by chain, side view.
PDB entry 6gna coloured by chain, top view.
Source organism: Clostridium acetobutylicum ATCC 824
Primary publication:
Ferredoxin-linked flavoenzyme defines a family of pyridine nucleotide-independent thioredoxin reductases.
Buey RM, Fernández-Justel D, de Pereda JM, Revuelta JL, Schürmann P, Buchanan BB, Balsera M
Proc Natl Acad Sci U S A 115 12967-12972 (2018)
PMID: 30510005

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188899 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD/NAD(P)-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 288 amino acids
Theoretical weight: 31.71 KDa
Source organism: Clostridium acetobutylicum ATCC 824
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97EM8 (Residues: 1-285; Coverage: 100%)
Gene name: CA_C3082
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase

Ligands and Environments

4 bound ligands:
Ligand FAD 1 x FAD
Ligand PGE 2 x PGE
Ligand PEG 3 x PEG
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: C2
Unit cell:
a: 83.221Å b: 40.942Å c: 81.093Å
α: 90° β: 103.76° γ: 90°
R-values:
R R work R free
0.177 0.175 0.201
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Redox and Thiols in Archaea.
Rawat et al. (2020)
1 more

1 mention without citation

Ferredoxin-linked flavoenzyme defines a family of pyridine nucleotide-independent thioredoxin reductases.
Buey et al. (2018)