› 6fhh

X-ray diffraction
2.7Å resolution

Crystal structure of bat influenza A/H17N10 polymerase with viral RNA promoter bound to a 22-mer modified Pol II CTD peptide with serine 5 thiophosphorylated.

Released:
DOI: 10.2210/pdb6fhh/pdb
PDB entry 6fhh coloured by chain, front view.
PDB entry 6fhh coloured by chain, side view.
PDB entry 6fhh coloured by chain, top view.
Source organisms:
Entry authors: Lukarska M, Cusack S

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-124814 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (6 distinct):
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 738 amino acids
Theoretical weight: 85.49 KDa
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM92 (Residues: 1-713; Coverage: 100%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 776 amino acids
Theoretical weight: 87.94 KDa
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM91 (Residues: 1-756; Coverage: 100%)
Gene name: PB1
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
Polymerase basic protein 2 Chain: C
Molecule details ›
Chain: C
Length: 809 amino acids
Theoretical weight: 91.03 KDa
Source organism: Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM90 (Residues: 1-760; Coverage: 100%)
Gene name: PB2
Sequence domains:
TYR-SER-PRO-THR-TPS-PRO-SER Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 22 amino acids
Theoretical weight: 2.63 KDa
Source organism: Homo sapiens
Expression system: Not provided
RNA (5'-R(*UP*AP*UP*AP*CP*CP*UP*CP*UP*GP*CP*UP*U)-3') Chain: R
Molecule details ›
Chain: R
Length: 18 nucleotides
Theoretical weight: 5.58 KDa
RNA (5'-R(P*AP*GP*UP*AP*GP*UP*AP*AP*CP*AP*AP*GP*AP*GP*GP*G)-3') Chain: V
Molecule details ›
Chain: V
Length: 16 nucleotides
Theoretical weight: 5.25 KDa

Ligands and Environments

2 bound ligands:
Ligand PO4 15 x PO4
Ligand ZN 1 x ZN
1 modified residue:
Ligand 2RX 6 x 2RX

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: C2
Unit cell:
a: 267.77Å b: 147.81Å c: 88.41Å
α: 90° β: 97.08° γ: 90°
R-values:
R R work R free
0.215 0.213 0.258
Expression systems:
  • Trichoplusia ni
  • Not provided

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