› 6ffh

X-ray diffraction
2.65Å resolution

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132987 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Metabotropic glutamate receptor 5 Chain: A
Molecule details ›
Chain: A
Length: 444 amino acids
Theoretical weight: 49.86 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41594 (Residues: 569-678, 680-836; Coverage: 22%)
  • Canonical: P00720 (Residues: 2-162; Coverage: 98%)
Gene names: E, GPRC1E, GRM5, MGLUR5
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand OLA 5 x OLA
Ligand MES 1 x MES
Ligand D7W 1 x D7W
Ligand OLC 1 x OLC
1 modified residue:
Ligand YCM 1 x YCM

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 143.217Å b: 43.478Å c: 82.383Å
α: 90° β: 99.22° γ: 90°
R-values:
R R work R free
0.245 0.244 0.267
Expression system: Spodoptera frugiperda

Quick links

Citations

7 review citations

Harnessing Ion-Binding Sites for GPCR Pharmacology.
Zarzycka et al. (2019)
6 more

15 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
14 more