› 6eu6

X-ray diffraction
1.98Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173001 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ammonium transporter Chain: A
Molecule details ›
Chain: A
Length: 405 amino acids
Theoretical weight: 43.46 KDa
Source organism: Candidatus Kuenenia stuttgartiensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1Q357 (Residues: 1-403; Coverage: 59%)
Gene names: KsCSTR_21800, amtb, kuste3690
Sequence domains: Ammonium Transporter Family

Ligands and Environments

5 bound ligands:
Ligand MES 2 x MES
Ligand ZDM 1 x ZDM
Ligand LMT 1 x LMT
Ligand NH4 2 x NH4
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P63
Unit cell:
a: 99.745Å b: 99.745Å c: 89.069Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.154 0.152 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Structural insights into the signalling mechanisms of two-component systems.
Jacob-Dubuisson et al. (2018)
8 more

1 mention without citation

Signaling ammonium across membranes through an ammonium sensor histidine kinase.
Pflüger et al. (2018)