› 6ech

X-ray diffraction
2.19Å resolution

Pyruvate Kinase Isoform L-type with phosphorylated Ser12 (pS12) in complex with FBP

Released:
DOI: 10.2210/pdb6ech/pdb
PDB entry 6ech coloured by chain, front view.
PDB entry 6ech coloured by chain, side view.
PDB entry 6ech coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production.
Gassaway BM, Cardone RL, Padyana AK, Petersen MC, Judd ET, Hayes S, Tong S, Barber KW, Apostolidi M, Abulizi A, Sheetz JB, Aerni HR, Gross S, Kung C, Samuel VT, Shulman GI, Kibbey RG, Rinehart J
Cell Rep 29 3394-3404.e9 (2019)
PMID: 31825824

Function and Biology Details

Reaction catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146378 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase PKLR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 550 amino acids
Theoretical weight: 59.91 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P12928 (Residues: 34-574; Coverage: 94%)
Gene name: Pklr
Sequence domains:

Ligands and Environments

9 bound ligands:
Ligand FBP 4 x FBP
Ligand MG 13 x MG
Ligand ATP 3 x ATP
Ligand OXL 4 x OXL
Ligand EDO 33 x EDO
Ligand PEG 2 x PEG
Ligand PG4 1 x PG4
Ligand PGE 1 x PGE
Ligand CL 1 x CL
1 modified residue:
Ligand SEP 4 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 86.55Å b: 108.9Å c: 296.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.238
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Multi-Tissue Acceleration of the Mitochondrial Phosphoenolpyruvate Cycle Improves Whole-Body Metabolic Health.
Abulizi et al. (2020)
3 more

1 mention without citation

Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production.
Gassaway et al. (2019)