› 6e9n

X-ray diffraction
2.92Å resolution

E. coli D-galactonate:proton symporter in the inward open form

Released:
DOI: 10.2210/pdb6e9n/pdb
PDB entry 6e9n coloured by chain, front view.
PDB entry 6e9n coloured by chain, side view.
PDB entry 6e9n coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structures suggest a mechanism for energy coupling by a family of organic anion transporters.
Leano JB, Batarni S, Eriksen J, Juge N, Pak JE, Kimura-Someya T, Robles-Colmenares Y, Moriyama Y, Stroud RM, Edwards RH
PLoS Biol 17 e3000260 (2019)
PMID: 31083648

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125366 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major facilitator superfamily (MFS) profile domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 460 amino acids
Theoretical weight: 50.5 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: J7QAK3 (Residues: 1-445; Coverage: 100%)
Gene names: A5U30_003247, B6R15_002606, BGM66_001314, C0P57_002075, C1Q91_001849, C2R31_003537, CF22_002085, CG831_001048, CTR35_001134, EN85_004672, FOI11_017180, FOI11_06000, G3V95_06840, G4A38_07235, G4A47_06635, G5603_13780, HEP34_000485, HHH44_002014, HJQ60_003343, HVW04_15475, HVY77_00060, I6H02_13960, J0541_001576, J5U05_002442, JNP96_00060, NEP60_13650, QDW62_00060, dgoT
Sequence domains: Sugar (and other) transporter

Ligands and Environments

2 bound ligands:
Ligand GCO 1 x GCO
Ligand BNG 5 x BNG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 76.7Å b: 107.63Å c: 103.21Å
α: 90° β: 108.32° γ: 90°
R-values:
R R work R free
0.246 0.243 0.297
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Molecular, Structural, Functional, and Pharmacological Sites for Vesicular Glutamate Transporter Regulation.
Pietrancosta et al. (2020)
2 more

11 mentions without citation

VGLUT substrates and inhibitors: A computational viewpoint.
Thompson et al. (2020)
10 more