› 6cmk

X-ray diffraction
1.73Å resolution

Crystal structure of Citrobacter koseri AztD

Released:
DOI: 10.2210/pdb6cmk/pdb
PDB entry 6cmk coloured by chain, front view.
PDB entry 6cmk coloured by chain, side view.
PDB entry 6cmk coloured by chain, top view.
Source organism: Citrobacter koseri ATCC BAA-895
Primary publication:
Crystal structures of AztD provide mechanistic insights into direct zinc transfer between proteins.
Neupane DP, Fullam SH, Chacón KN, Yukl ET
Commun Biol 2 308 (2019)
PMID: 31428696

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108553 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Zinc chaperone AztD Chains: A, B
Molecule details ›
Chains: A, B
Length: 421 amino acids
Theoretical weight: 45.47 KDa
Source organism: Citrobacter koseri ATCC BAA-895
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8AF35 (Residues: 1-421; Coverage: 100%)
Gene names: CKO_00948, aztD

Ligands and Environments

5 bound ligands:
Ligand MES 2 x MES
Ligand ZN 4 x ZN
Ligand EDO 4 x EDO
Ligand PGE 1 x PGE
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 54.23Å b: 128.79Å c: 56.85Å
α: 90° β: 105.2° γ: 90°
R-values:
R R work R free
0.154 0.153 0.188
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Regulation of zinc-dependent enzymes by metal carrier proteins.
Thompson MW. (2022)
4 other articles

1 mention without citation

Crystal structures of AztD provide mechanistic insights into direct zinc transfer between proteins.
Neupane et al. (2019)