› 6bqz

X-ray diffraction
2.3Å resolution

Crystal Structure of Tyrosine-tRNA Synthetase from Acinetobacter baumannii with bound L-Tyrosine

Released:
DOI: 10.2210/pdb6bqz/pdb
PDB entry 6bqz coloured by chain, front view.
PDB entry 6bqz coloured by chain, side view.
PDB entry 6bqz coloured by chain, top view.
Source organism: Acinetobacter baumannii
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109250 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 408 amino acids
Theoretical weight: 45.3 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B0VML7 (Residues: 5-404; Coverage: 99%)
Gene names: ABSDF0019, tyrS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains: HUPs

Ligands and Environments

3 bound ligands:
Ligand TYR 2 x TYR
Ligand MG 2 x MG
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P1
Unit cell:
a: 48.26Å b: 49.8Å c: 73.37Å
α: 87.65° β: 107.11° γ: 113.86°
R-values:
R R work R free
0.184 0.18 0.231
Expression system: Escherichia coli BL21(DE3)

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