Function and Biology Details
Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
ORF1ab and Ubiquitin-like protein ISG15 (preferred)
PDBe Complex ID:
PDB-CPX-125467 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ORF1ab
Molecule details ›
Chains: A, B
Length: 259 amino acids
Theoretical weight: 28.88 KDa
Source organism: Human betacoronavirus 2c EMC/2012
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Coronavirus papain-like peptidase
Structure domains:
Length: 259 amino acids
Theoretical weight: 28.88 KDa
Source organism: Human betacoronavirus 2c EMC/2012
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
K0BWD0 (Residues: 1544-1801; Coverage: 4%)
Sequence domains: Coronavirus papain-like peptidase
Structure domains:
Ubiquitin-like protein ISG15
Molecule details ›
Chains: C, D
Length: 156 amino acids
Theoretical weight: 17.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Length: 156 amino acids
Theoretical weight: 17.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P05161 (Residues: 1-156; Coverage: 95%)
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
