› 6b5k

X-ray diffraction
1.6Å resolution

Mycobacterium tuberculosis RmlA in complex with Mg/dTTP

Released:
DOI: 10.2210/pdb6b5k/pdb
PDB entry 6b5k coloured by chain, front view.
PDB entry 6b5k coloured by chain, side view.
PDB entry 6b5k coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
The structure of glucose-1-phosphate thymidylyltransferase from Mycobacterium tuberculosis reveals the location of an essential magnesium ion in the RmlA-type enzymes.
Brown HA, Thoden JB, Tipton PA, Holden HM
Protein Sci 27 441-450 (2018)
PMID: 29076563

Function and Biology Details

Reaction catalysed: 
dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161538 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-1-phosphate thymidylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 32.59 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WH13 (Residues: 1-288; Coverage: 100%)
Gene names: Rv0334, rfbA, rmlA
Sequence domains: Nucleotidyl transferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

4 bound ligands:
Ligand TTP 2 x TTP
Ligand MG 4 x MG
Ligand TYD 2 x TYD
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I4
Unit cell:
a: 96.403Å b: 96.403Å c: 151.924Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.195 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Pangenome Analysis of Mycobacterium tuberculosis Reveals Core-Drug Targets and Screening of Promising Lead Compounds for Drug Discovery.
Dar et al. (2020)
4 more

1 mention without citation

The structure of glucose-1-phosphate thymidylyltransferase from Mycobacterium tuberculosis reveals the location of an essential magnesium ion in the RmlA-type enzymes.
Brown et al. (2018)