6qib

X-ray diffraction
2.8Å resolution

The crystal structure of Pol2CORE in complex with DNA and an incoming nucleotide, carrying an Fe-S cluster

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-120491 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase epsilon catalytic subunit A Chain: A
Molecule details ›
Chain: A
Length: 1192 amino acids
Theoretical weight: 137.2 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21951 (Residues: 1-1187; Coverage: 53%)
Gene names: DUN2, N0825, POL2, YNL262W
Sequence domains:
Primer_11ddC Chain: P
Molecule details ›
Chain: P
Length: 11 nucleotides
Theoretical weight: 3.29 KDa
Source organism: synthetic construct
Expression system: Not provided
Template16 Chain: T
Molecule details ›
Chain: T
Length: 16 nucleotides
Theoretical weight: 4.89 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: C2
Unit cell:
a: 158.36Å b: 70.402Å c: 154.24Å
α: 90° β: 112.99° γ: 90°
R-values:
R R work R free
0.226 0.223 0.286
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided