› 6mrj

X-ray diffraction
2.8Å resolution

Crystal structure of H.pylori NikR in complex with DNA

Released:
DOI: 10.2210/pdb6mrj/pdb
PDB entry 6mrj coloured by chain, front view.
PDB entry 6mrj coloured by chain, side view.
PDB entry 6mrj coloured by chain, top view.
Source organisms:
Entry authors: Pozharski E, de Serrano V

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120303 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Putative nickel-responsive regulator Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 148 amino acids
Theoretical weight: 17.17 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: O25896 (Residues: 1-148; Coverage: 100%)
Gene name: HP_1338
Sequence domains:
DNA (36-MER) Chain: L
Molecule details ›
Chain: L
Length: 36 nucleotides
Theoretical weight: 11.13 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (36-MER) Chain: M
Molecule details ›
Chain: M
Length: 36 nucleotides
Theoretical weight: 11.01 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NI 4 x NI
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 72.37Å b: 94.18Å c: 204.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.241
Expression systems:
  • Escherichia coli
  • Not provided

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