› 6lb3

X-ray diffraction
2.5Å resolution

Crystal structure of PA4674 in complex with its operator DNA (18bp) from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb6lb3/pdb
PDB entry 6lb3 coloured by chain, front view.
PDB entry 6lb3 coloured by chain, side view.
PDB entry 6lb3 coloured by chain, top view.
Source organisms:
Entry authors: Liu Y, Zhang H, Gao Z, Dong Y

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120207 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH cro/C1-type domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 109 amino acids
Theoretical weight: 12.23 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HVC1 (Residues: 1-101; Coverage: 100%)
Gene name: PA4674
Sequence domains: Helix-turn-helix
DNA (5'-D(P*AP*CP*GP*CP*TP*TP*AP*AP*CP*GP*TP*TP*AP*AP*GP*GP*GP*T)-3') Chains: I, K, M
Molecule details ›
Chains: I, K, M
Length: 18 nucleotides
Theoretical weight: 5.56 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Not provided
DNA (5'-D(P*AP*CP*CP*CP*TP*TP*AP*AP*CP*GP*TP*TP*AP*AP*GP*CP*GP*T)-3') Chains: J, L, N
Molecule details ›
Chains: J, L, N
Length: 18 nucleotides
Theoretical weight: 5.48 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P21
Unit cell:
a: 57.284Å b: 95.57Å c: 128.857Å
α: 90° β: 96.29° γ: 90°
R-values:
R R work R free
0.219 0.218 0.262
Expression systems:
  • Escherichia coli
  • Not provided

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