› 6gy3

X-ray diffraction
2.68Å resolution

Crystal Structure of C. glutamicum AmtR bound to glnA operator DNA

Released:
DOI: 10.2210/pdb6gy3/pdb
PDB entry 6gy3 coloured by chain, front view.
PDB entry 6gy3 coloured by chain, side view.
PDB entry 6gy3 coloured by chain, top view.
Source organism: Corynebacterium glutamicum
Entry authors: Sevvana M, Muller YA

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119974 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 202 amino acids
Theoretical weight: 22.33 KDa
Source organism: Corynebacterium glutamicum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79VH8 (Residues: 19-220; Coverage: 91%)
Gene name: Cgl0863
Sequence domains: Bacterial regulatory proteins, tetR family
DNA (5'-D(*GP*TP*CP*TP*AP*TP*AP*GP*AP*TP*CP*GP*AP*TP*AP*GP*AP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 18 nucleotides
Theoretical weight: 5.54 KDa
Source organism: Corynebacterium glutamicum
Expression system: Not provided
DNA (5'-D(*GP*TP*CP*TP*AP*TP*CP*GP*AP*TP*CP*TP*AP*TP*AP*GP*AP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.49 KDa
Source organism: Corynebacterium glutamicum
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 63.32Å b: 76.15Å c: 117.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.251
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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