› 6fn0

X-ray diffraction
2.9Å resolution

The animal-like Cryptochrome from Chlamydomonas reinhardtii in complex with 6-4 DNA

Released:
DOI: 10.2210/pdb6fn0/pdb
PDB entry 6fn0 coloured by chain, front view.
PDB entry 6fn0 coloured by chain, side view.
PDB entry 6fn0 coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Structure of the bifunctional cryptochrome aCRY from Chlamydomonas reinhardtii.
Franz S, Ignatz E, Wenzel S, Zielosko H, Putu EPGN, Maestre-Reyna M, Tsai MD, Yamamoto J, Mittag M, Essen LO
Nucleic Acids Res 46 8010-8022 (2018)
PMID: 30032195

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108694 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Cryptochrome photoreceptor Chain: A
Molecule details ›
Chain: A
Length: 499 amino acids
Theoretical weight: 56.59 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8J8W0 (Residues: 1-496; Coverage: 83%)
Gene name: CHLRE_06g278251v5
Sequence domains:
Structure domains:
DNA (5'-D(*CP*AP*GP*CP*GP*GP*(64T)P*(5PY)P*GP*CP*CP*GP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.31 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided
DNA (5'-D(*CP*AP*CP*GP*GP*CP*AP*AP*CP*CP*GP*CP*TP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 14 nucleotides
Theoretical weight: 4.25 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided

Ligands and Environments

6 bound ligands:
Ligand FAD 1 x FAD
Ligand MES 1 x MES
Ligand GOL 1 x GOL
Ligand MG 2 x MG
Ligand CL 1 x CL
Ligand 1PE 1 x 1PE
2 modified residues:
Ligand 64T 1 x 64T
Ligand 5PY 1 x 5PY

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P42212
Unit cell:
a: 146.24Å b: 146.24Å c: 67.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.212
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Diatom Molecular Research Comes of Age: Model Species for Studying Phytoplankton Biology and Diversity.
Falciatore et al. (2020)
3 more

2 mentions without citation

Structure of the bifunctional cryptochrome aCRY from Chlamydomonas reinhardtii.
Franz et al. (2018)
1 more