› 6akp

X-ray diffraction
2.32Å resolution

Crystal Structural of FOXC2 DNA binding domain bound to PC promoter

Released:
DOI: 10.2210/pdb6akp/pdb
PDB entry 6akp coloured by chain, front view.
PDB entry 6akp coloured by chain, side view.
PDB entry 6akp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for DNA recognition by FOXC2.
Chen X, Wei H, Li J, Liang X, Dai S, Jiang L, Guo M, Qu L, Chen Z, Chen L, Chen Y
Nucleic Acids Res 47 3752-3764 (2019)
PMID: 30722065

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119560 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Forkhead box protein C2 Chain: C
Molecule details ›
Chain: C
Length: 103 amino acids
Theoretical weight: 12.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99958 (Residues: 72-172; Coverage: 20%)
Gene names: FKHL14, FOXC2, MFH1
Sequence domains: Forkhead domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(AP*CP*AP*CP*AP*AP*AP*TP*AP*TP*TP*TP*GP*TP*GP*T)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 41.837Å b: 80.214Å c: 101.154Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.247
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Metastasis and MAPK Pathways.
Kciuk et al. (2022)
2 more

5 mentions without citation

Toward a mechanistic understanding of DNA binding by forkhead transcription factors and its perturbation by pathogenic mutations.
Dai et al. (2021)
4 more