› 5zq8

X-ray diffraction
2.18Å resolution

Crystal structure of spRlmCD with U747 stemloop RNA

Released:
DOI: 10.2210/pdb5zq8/pdb
PDB entry 5zq8 coloured by chain, front view.
PDB entry 5zq8 coloured by chain, side view.
PDB entry 5zq8 coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Unveiling the structural features that determine the dual methyltransferase activities of Streptococcus pneumoniae RlmCD.
Jiang Y, Yu H, Li F, Cheng L, Zhu L, Shi Y, Gong Q
PLoS Pathog 14 e1007379 (2018)
PMID: 30388185

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188960 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Uncharacterized RNA methyltransferase SP_1029 Chains: A, B
Molecule details ›
Chains: A, B
Length: 454 amino acids
Theoretical weight: 51.39 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q97R12 (Residues: 1-454; Coverage: 84%)
Gene name: SP_1029
Sequence domains:
RNA (5'-R(*CP*CP*GP*UP*(MUM)P*GP*AP*AP*AP*AP*GP*G)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 12 nucleotides
Theoretical weight: 3.88 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SAH 2 x SAH
Ligand NI 2 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P1
Unit cell:
a: 62.23Å b: 62.259Å c: 79.818Å
α: 74.58° β: 85.47° γ: 65.03°
R-values:
R R work R free
0.248 0.245 0.303
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

Quick links

Citations

1 mention without citation

Unveiling the structural features that determine the dual methyltransferase activities of Streptococcus pneumoniae RlmCD.
Jiang et al. (2018)