› 5yz2

X-ray diffraction
1.75Å resolution

the cystathionine-beta-synthase (CBS) domain of magnesium and cobalt efflux protein CorC in complex with both C2'- and C3'-endo AMP

Released:
DOI: 10.2210/pdb5yz2/pdb
PDB entry 5yz2 coloured by chain, front view.
PDB entry 5yz2 coloured by chain, side view.
PDB entry 5yz2 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The C2'- and C3'-endo equilibrium for AMP molecules bound in the cystathionine-beta-synthase domain.
Feng N, Qi C, Hou YJ, Zhang Y, Wang DC, Li DF
Biochem Biophys Res Commun 497 646-651 (2018)
PMID: 29453981

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142415 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Magnesium and cobalt efflux protein CorC Chains: A, B
Molecule details ›
Chains: A, B
Length: 144 amino acids
Theoretical weight: 16.72 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AE78 (Residues: 67-193; Coverage: 44%)
Gene names: JW0655, b0658, corC, ybeX
Sequence domains: CBS domain
Structure domains: CBS domain Like

Ligands and Environments

1 bound ligand:
Ligand AMP 2 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 53.898Å b: 56.29Å c: 54.349Å
α: 90° β: 114.73° γ: 90°
R-values:
R R work R free
0.211 0.209 0.236
Expression system: Escherichia coli BL21(DE3)

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Citations

2 mentions without citation

Current Structural Knowledge on the CNNM Family of Magnesium Transport Mediators.
Giménez-Mascarell et al. (2019)
1 more