› 5yyc

X-ray diffraction
1.8Å resolution

Crystal structure of alanine racemase from Bacillus pseudofirmus (OF4)

Released:
DOI: 10.2210/pdb5yyc/pdb
PDB entry 5yyc coloured by chain, front view.
PDB entry 5yyc coloured by chain, side view.
PDB entry 5yyc coloured by chain, top view.
Source organism: Alkalihalophilus pseudofirmus OF4
Primary publication:
Structural features and kinetic characterization of alanine racemase from Bacillus pseudofirmus OF4.
Dong H, Hu T, He G, Lu D, Qi J, Dou Y, Long W, He X, Ju J, Su D
Biochem Biophys Res Commun 497 139-145 (2018)
PMID: 29427660

Function and Biology Details

Reaction catalysed: 
L-alanine = D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine racemase Chains: A, C
Molecule details ›
Chains: A, C
Length: 369 amino acids
Theoretical weight: 40.37 KDa
Source organism: Alkalihalophilus pseudofirmus OF4
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: D3FSP1 (Residues: 1-369; Coverage: 100%)
Gene names: BpOF4_09770, alr
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 97.301Å b: 97.314Å c: 112.569Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.149 0.174
Expression system: Escherichia coli K-12

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Citations

1 review citation

Advances in Key Drug Target Identification and New Drug Development for Tuberculosis.
Mi et al. (2022)
1 other article