› 5yq7

Electron Microscopy
4.1Å resolution

Cryo-EM structure of the RC-LH core complex from Roseiflexus castenholzii

Released:
DOI: 10.2210/pdb5yq7/pdb
PDB entry 5yq7 coloured by chain, front view.
PDB entry 5yq7 coloured by chain, side view.
PDB entry 5yq7 coloured by chain, top view.
Source organism: Roseiflexus castenholzii
Primary publication:
Cryo-EM structure of the RC-LH core complex from an early branching photosynthetic prokaryote.
Xin Y, Shi Y, Niu T, Wang Q, Niu W, Huang X, Ding W, Yang L, Blankenship RE, Xu X, Sun F
Nat Commun 9 1568 (2018)
PMID: 29674684
Related structures: EMD-6828

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 35-mer (preferred)
PDBe Complex ID:
PDB-CPX-166344 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Antenna complex alpha/beta subunit domain-containing protein Chains: 0, 2, 4, 6, 8, B, E, G, I, K, O, Q, S, U, W
Molecule details ›
Chains: 0, 2, 4, 6, 8, B, E, G, I, K, O, Q, S, U, W
Length: 55 amino acids
Theoretical weight: 6.43 KDa
Source organism: Roseiflexus castenholzii
UniProt:
  • Canonical: Q83XD2 (Residues: 1-55; Coverage: 100%)
Gene name: pufB
Sequence domains: Antenna complex alpha/beta subunit
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 310 amino acids
Theoretical weight: 34.43 KDa
Source organism: Roseiflexus castenholzii
UniProt:
  • Canonical: Q83XD0 (Residues: 1-310; Coverage: 48%)
Gene name: pufLM
Sequence domains: Photosynthetic reaction centre protein
Cytochrome subunit of photosynthetic reaction center Chain: C
Molecule details ›
Chain: C
Length: 320 amino acids
Theoretical weight: 34.92 KDa
Source organism: Roseiflexus castenholzii
UniProt:
  • Canonical: Q83XC9 (Residues: 1-320; Coverage: 100%)
Gene name: pufC
Sequence domains: Photosynthetic reaction centre cytochrome C subunit
Alpha subunit of light-harvesting 1 Chains: 1, 3, 5, 7, 9, A, D, F, H, J, N, P, R, T, V
Molecule details ›
Chains: 1, 3, 5, 7, 9, A, D, F, H, J, N, P, R, T, V
Length: 42 amino acids
Theoretical weight: 4.72 KDa
Source organism: Roseiflexus castenholzii
UniProt:
  • Canonical: Q83XD1 (Residues: 1-42; Coverage: 100%)
Gene name: pufA
Peptide from Precursor for L and M subunits of photosynthetic reaction center Chain: Y
Molecule details ›
Chain: Y
Length: 25 amino acids
Theoretical weight: 2.15 KDa
Source organism: Roseiflexus castenholzii
Subunit X Chain: X
Molecule details ›
Chain: X
Length: 23 amino acids
Theoretical weight: 1.98 KDa
Source organism: Roseiflexus castenholzii
Reaction center protein L chain Chain: M
Molecule details ›
Chain: M
Length: 306 amino acids
Theoretical weight: 34.95 KDa
Source organism: Roseiflexus castenholzii
UniProt:
  • Canonical: Q83XD0 (Residues: 336-641; Coverage: 48%)
Gene name: pufLM
Sequence domains: Photosynthetic reaction centre protein

Ligands and Environments

6 bound ligands:
Ligand BCL 48 x BCL
Ligand BPH 3 x BPH
Ligand MQE 2 x MQE
Ligand FE 1 x FE
Ligand HEM 4 x HEM
Ligand KGD 14 x KGD
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-6828

Quick links

Citations

2 review citations

Recent Advances in Developing Artificial Autotrophic Microorganism for Reinforcing CO2 Fixation.
Liang et al. (2020)
1 more

6 mentions without citation

A comparative look at structural variation among RC-LH1 'Core' complexes present in anoxygenic phototrophic bacteria.
Gardiner et al. (2020)
5 more