› 5yhf

X-ray diffraction
2.8Å resolution

Crystal structure of SecDF in Super-membrane-facing form

Released:
DOI: 10.2210/pdb5yhf/pdb
PDB entry 5yhf coloured by chain, front view.
PDB entry 5yhf coloured by chain, side view.
PDB entry 5yhf coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Remote Coupled Drastic β-Barrel to β-Sheet Transition of the Protein Translocation Motor.
Furukawa A, Nakayama S, Yoshikaie K, Tanaka Y, Tsukazaki T
Structure 26 485-489.e2 (2018)
PMID: 29398525

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein translocase subunit SecDF Chain: A
Molecule details ›
Chain: A
Length: 741 amino acids
Theoretical weight: 80.59 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SKE6 (Residues: 1-735; Coverage: 100%)
Gene names: TTHA0697, secDF
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand OLC 5 x OLC
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P1
Unit cell:
a: 55.19Å b: 65.64Å c: 79.98Å
α: 75.11° β: 75.23° γ: 80.83°
R-values:
R R work R free
0.207 0.203 0.246
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Pushing the Envelope: The Mysterious Journey Through the Bacterial Secretory Machinery, and Beyond.
Troman et al. (2021)
1 more

5 mentions without citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
4 more