› 5xte

Electron Microscopy
3.4Å resolution

Cryo-EM structure of human respiratory complex III (cytochrome bc1 complex)

Released:
DOI: 10.2210/pdb5xte/pdb
PDB entry 5xte coloured by chain, front view.
PDB entry 5xte coloured by chain, side view.
PDB entry 5xte coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Architecture of Human Mitochondrial Respiratory Megacomplex I2III2IV2.
Guo R, Zong S, Wu M, Gu J, Yang M
Cell 170 1247-1257.e12 (2017)
PMID: 28844695
Related structures: EMD-6774

Function and Biology Details

Reaction catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 22-mer (preferred)
PDBe Complex ID:
PDB-CPX-127221 (preferred)
Entry contents:
11 distinct polypeptide molecules
Macromolecules (11 distinct):
Cytochrome b-c1 complex subunit 8 Chains: A, N
Molecule details ›
Chains: A, N
Length: 81 amino acids
Theoretical weight: 9.79 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O14949 (Residues: 2-82; Coverage: 99%)
Gene name: UQCRQ
Sequence domains: UcrQ family
Structure domains: Cytochrome b-c1 complex subunit 8
Cytochrome b-c1 complex subunit 9 Chains: B, O
Molecule details ›
Chains: B, O
Length: 57 amino acids
Theoretical weight: 5.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P47985 (Residues: 1-57; Coverage: 21%)
Gene name: UQCRFS1
Sequence domains: Ubiquinol-cytochrome c reductase 8 kDa, N-terminal
Structure domains: Cytochrome Bc1 Complex; Chain I
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chains: C, P
Molecule details ›
Chains: C, P
Length: 196 amino acids
Theoretical weight: 21.65 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P47985 (Residues: 79-274; Coverage: 72%)
Gene name: UQCRFS1
Sequence domains:
Structure domains:
Cytochrome b-c1 complex subunit 9 Chains: D, Q
Molecule details ›
Chains: D, Q
Length: 62 amino acids
Theoretical weight: 7.19 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9UDW1 (Residues: 2-63; Coverage: 98%)
Gene names: HSPC119, UCRC, UQCR10
Sequence domains: Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like
Structure domains: Cytochrome b-c1 complex subunit 9
Cytochrome b-c1 complex subunit 6, mitochondrial Chains: E, R
Molecule details ›
Chains: E, R
Length: 75 amino acids
Theoretical weight: 8.99 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P07919 (Residues: 17-91; Coverage: 82%)
Gene name: UQCRH
Sequence domains: Ubiquinol-cytochrome C reductase hinge protein
Structure domains: Ubiquinol-cytochrome C reductase hinge domain
Cytochrome b-c1 complex subunit 7 Chains: F, S
Molecule details ›
Chains: F, S
Length: 106 amino acids
Theoretical weight: 13.04 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P14927 (Residues: 6-111; Coverage: 96%)
Gene names: UQBP, UQCRB
Sequence domains: Ubiquinol-cytochrome C reductase complex 14kD subunit
Structure domains: Cytochrome b-c1 complex subunit 7
Cytochrome b-c1 complex subunit 10 Chains: G, T
Molecule details ›
Chains: G, T
Length: 51 amino acids
Theoretical weight: 5.96 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O14957 (Residues: 2-52; Coverage: 91%)
Gene names: UQCR, UQCR11
Sequence domains: Ubiquinol-cytochrome C reductase complex, 6.4kD protein
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Cytochrome c1, heme protein, mitochondrial Chains: H, U
Molecule details ›
Chains: H, U
Length: 241 amino acids
Theoretical weight: 27.39 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P08574 (Residues: 85-325; Coverage: 74%)
Gene name: CYC1
Sequence domains: Cytochrome C1 family
Structure domains:
Cytochrome b Chains: J, V
Molecule details ›
Chains: J, V
Length: 378 amino acids
Theoretical weight: 42.54 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P00156 (Residues: 2-379; Coverage: 100%)
Gene names: COB, CYTB, MT-CYB, MTCYB
Sequence domains:
Structure domains: Cytochrome Bc1 Complex; Chain C
Cytochrome b-c1 complex subunit 2, mitochondrial Chains: K, W
Molecule details ›
Chains: K, W
Length: 419 amino acids
Theoretical weight: 44.95 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P22695 (Residues: 35-453; Coverage: 93%)
Gene name: UQCRC2
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like
Cytochrome b-c1 complex subunit 1, mitochondrial Chains: L, Y
Molecule details ›
Chains: L, Y
Length: 446 amino acids
Theoretical weight: 49.18 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P31930 (Residues: 35-480; Coverage: 93%)
Gene name: UQCRC1
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like

Ligands and Environments

6 bound ligands:
Ligand CDL 9 x CDL
Ligand FES 2 x FES
Ligand PEE 6 x PEE
Ligand HEC 2 x HEC
Ligand HEM 4 x HEM
Ligand PLX 3 x PLX
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-6774

Quick links

Citations

57 review citations

The multifaceted role of iron in renal health and disease.
van Swelm et al. (2020)
56 more

22 mentions without citation

Maturation of the respiratory complex II flavoprotein.
Sharma et al. (2019)
21 more