› 5xoo

X-ray diffraction
2.85Å resolution

The structure of hydra Fam20 with sugar

Released:
DOI: 10.2210/pdb5xoo/pdb
PDB entry 5xoo coloured by chain, front view.
PDB entry 5xoo coloured by chain, side view.
PDB entry 5xoo coloured by chain, top view.
Source organism: Hydra vulgaris
Primary publication:
Structure and evolution of the Fam20 kinases.
Zhang H, Zhu Q, Cui J, Wang Y, Chen MJ, Guo X, Tagliabracci VS, Dixon JE, Xiao J
Nat Commun 9 1218 (2018)
PMID: 29572475

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197515 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
FAM20 C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 393 amino acids
Theoretical weight: 45.32 KDa
Source organism: Hydra vulgaris
Expression system: Trichoplusia ni
UniProt:
  • Canonical: T2MHS6 (Residues: 24-415; Coverage: 95%)
Gene names: FAM20B, LOC100203383
Sequence domains: Golgi casein kinase, C-terminal, Fam20

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP, GAL
Carbohydrate polymer
3 bound ligands:
Ligand NAG 4 x NAG
Ligand MBN 1 x MBN
Ligand ADN 2 x ADN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P212121
Unit cell:
a: 80.601Å b: 123.58Å c: 143.936Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.2 0.217
Expression system: Trichoplusia ni

Quick links

Citations

6 review citations

Prospects for pharmacological targeting of pseudokinases.
Kung et al. (2019)
5 more

3 mentions without citation

Thinking outside of the cell: Secreted protein kinases in bacteria, parasites, and mammals.
Park et al. (2019)
2 more