› 5xc5

X-ray diffraction
1.4Å resolution

Crystal structure of Acanthamoeba polyphaga mimivirus Rab GTPase in complex with GTP

Released:
DOI: 10.2210/pdb5xc5/pdb
PDB entry 5xc5 coloured by chain, front view.
PDB entry 5xc5 coloured by chain, side view.
PDB entry 5xc5 coloured by chain, top view.
Source organism: Acanthamoeba polyphaga mimivirus
Primary publication:
Crystal structures of two forms of the Acanthamoeba polyphaga mimivirus Rab GTPase.
Ku B, You JA, Oh KJ, Yun HY, Lee HS, Shin HC, Jung J, Shin YB, Kim SJ
Arch Virol 162 3407-3416 (2017)
PMID: 28779233

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178383 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable Rab-related GTPase Chain: A
Molecule details ›
Chain: A
Length: 176 amino acids
Theoretical weight: 20.19 KDa
Source organism: Acanthamoeba polyphaga mimivirus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5UQ27 (Residues: 9-184; Coverage: 82%)
Gene name: MIMI_R214
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P212121
Unit cell:
a: 32.924Å b: 53.724Å c: 96.482Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.19
Expression system: Escherichia coli

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Citations

5 citation in other articles

Evolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes.
Ramrath et al. (2018)
4 more