5x2p

X-ray diffraction
2.61Å resolution

Crystal structure of the medaka fish taste receptor T1r2a-T1r3 ligand binding domains in complex with L-glutamate

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-207369 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
G-protein coupled receptors family 3 profile domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 461 amino acids
Theoretical weight: 51.47 KDa
Source organism: Oryzias latipes
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: A0A173M0G2 (Residues: 12-466; Coverage: 57%)
Gene name: TAS1R2a
Sequence domains: Receptor family ligand binding region
Taste receptor type 1 member 3 Chains: B, D
Molecule details ›
Chains: B, D
Length: 478 amino acids
Theoretical weight: 53.13 KDa
Source organism: Oryzias latipes
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: A0A173M094 (Residues: 12-483; Coverage: 57%)
Gene name: TAS1R3
Sequence domains: Receptor family ligand binding region
Fab16A Heavy chain Chains: H, J
Molecule details ›
Chains: H, J
Length: 225 amino acids
Theoretical weight: 24.18 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Fab16A Light chain Chains: K, L
Molecule details ›
Chains: K, L
Length: 217 amino acids
Theoretical weight: 23.9 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 99.632Å b: 116.063Å c: 129.592Å
α: 90° β: 91.56° γ: 90°
R-values:
R R work R free
0.157 0.153 0.228
Expression systems:
  • Drosophila melanogaster
  • Mus musculus