› 5wt2

X-ray diffraction
2.3Å resolution

Function and Biology Details

Reaction catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127699 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase IscS Chain: A
Molecule details ›
Chain: A
Length: 401 amino acids
Theoretical weight: 44.13 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: O25008 (Residues: 1-387; Coverage: 100%)
Gene names: HP_0220, iscS
Sequence domains: Aminotransferase class-V

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
2 bound ligands:
Ligand IPA 1 x IPA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P41212
Unit cell:
a: 103.01Å b: 103.01Å c: 133.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.215 0.239
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Fe-S cluster biogenesis by the bacterial Suf pathway.
Blahut et al. (2020)
2 more

5 mentions without citation

Radical SAM Enzymes and Metallocofactor Assembly: A Structural Point of View.
Nicolet et al. (2022)
4 more