› 5w7r

X-ray diffraction
2.5Å resolution

Crystal structure of OxaC in complex with SAH and oxaline

Released:
DOI: 10.2210/pdb5w7r/pdb
PDB entry 5w7r coloured by chain, front view.
PDB entry 5w7r coloured by chain, side view.
PDB entry 5w7r coloured by chain, top view.
Source organism: Penicillium oxalicum
Primary publication:
Unveiling sequential late-stage methyltransferase reactions in the meleagrin/oxaline biosynthetic pathway.
Newmister SA, Romminger S, Schmidt JJ, Williams RM, Smith JL, Berlinck RGS, Sherman DH
Org Biomol Chem 16 6450-6459 (2018)
PMID: 30141817

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103265 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-methyltransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 429 amino acids
Theoretical weight: 47.28 KDa
Source organism: Penicillium oxalicum
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1B2TT09 (Residues: 1-405; Coverage: 100%)
Sequence domains: O-methyltransferase domain

Ligands and Environments

2 bound ligands:
Ligand 9Z7 1 x 9Z7
Ligand SAH 1 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I422
Unit cell:
a: 162.878Å b: 162.878Å c: 90.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.219 0.25
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Diversity of the reaction mechanisms of SAM-dependent enzymes.
Sun et al. (2021)
2 more

1 mention without citation

Unveiling sequential late-stage methyltransferase reactions in the meleagrin/oxaline biosynthetic pathway.
Newmister et al. (2018)