› 5vn7

X-ray diffraction
2.7Å resolution

Structure of bacteriorhodopsin from crystals grown at 20 deg Celcius using GlyNCOC15+4 as an LCP host lipid

Released:
DOI: 10.2210/pdb5vn7/pdb
PDB entry 5vn7 coloured by chain, front view.
PDB entry 5vn7 coloured by chain, side view.
PDB entry 5vn7 coloured by chain, top view.
Source organism: Halobacterium salinarum NRC-1
Primary publication:
Chemically Stable Lipids for Membrane Protein Crystallization.
Ishchenko A, Peng L, Zinovev E, Vlasov A, Lee SC, Kuklin A, Mishin A, Borshchevskiy V, Zhang Q, Cherezov V
Cryst Growth Des 17 3502-3511 (2017)
PMID: 29290736

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 28.54 KDa
Source organism: Halobacterium salinarum NRC-1
Expression system: Halobacterium salinarum
UniProt:
  • Canonical: P02945 (Residues: 1-262; Coverage: 100%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

No bound ligands
1 modified residue:
Ligand LYR 2 x LYR

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: R3
Unit cell:
a: 107.05Å b: 107.05Å c: 149.86Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.196 0.225
Expression system: Halobacterium salinarum

Quick links

Citations

4 review citations

Serial Femtosecond Crystallography of G Protein-Coupled Receptors.
Stauch et al. (2018)
3 more

1 mention without citation

Chemically Stable Lipids for Membrane Protein Crystallization.
Ishchenko et al. (2017)