› 5vja

X-ray diffraction
2.46Å resolution

Crystal Structure of human zipper-interacting protein kinase (ZIPK, alias DAPK3) in complex with a pyrazolo[3,4-d]pyrimidinone ligand (HS38)

Released:
DOI: 10.2210/pdb5vja/pdb
PDB entry 5vja coloured by chain, front view.
PDB entry 5vja coloured by chain, side view.
PDB entry 5vja coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Targeting Pim Kinases and DAPK3 to Control Hypertension.
Carlson DA, Singer MR, Sutherland C, Redondo C, Alexander LT, Hughes PF, Knapp S, Gurley SB, Sparks MA, MacDonald JA, Haystead TAJ
Cell Chem Biol 25 1195-1207.e32 (2018)
PMID: 30033129

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128887 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Death-associated protein kinase 3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 283 amino acids
Theoretical weight: 32.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43293 (Residues: 9-289; Coverage: 62%)
Gene names: DAPK3, ZIPK
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand DMS 4 x DMS
Ligand DUK 4 x DUK
Ligand ILE 1 x ILE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 53.492Å b: 98.491Å c: 115.022Å
α: 90° β: 90.07° γ: 90°
R-values:
R R work R free
0.19 0.188 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Novel Functions of Death-Associated Protein Kinases through Mitogen-Activated Protein Kinase-Related Signals.
Elbadawy et al. (2018)
3 more

1 mention without citation

Targeting Pim Kinases and DAPK3 to Control Hypertension.
Carlson et al. (2018)