› 5ver

X-ray diffraction
2.81Å resolution

MOUSE KYNURENINE AMINOTRANSFERASE III, RE-REFINEMENT OF THE PDB STRUCTURE 3E2Z

Released:
DOI: 10.2210/pdb5ver/pdb
PDB entry 5ver coloured by chain, front view.
PDB entry 5ver coloured by chain, side view.
PDB entry 5ver coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Detect, correct, retract: How to manage incorrect structural models.
Wlodawer A, Dauter Z, Porebski PJ, Minor W, Stanfield R, Jaskolski M, Pozharski E, Weichenberger CX, Rupp B
FEBS J 285 444-466 (2018)
PMID: 29113027

Function and Biology Details

Reactions catalysed: 
(1a) L-kynurenine + glyoxylate = 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine 
(1a) L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
(1a) an L-cysteine-S-conjugate = a thiol + 2-aminoporp-2-enoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-180753 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kynurenine--oxoglutarate transaminase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 410 amino acids
Theoretical weight: 46.22 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q71RI9 (Residues: 42-451; Coverage: 90%)
Gene names: Ccbl2, Kat3, Kyat3
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PMP 1 x PMP

Cofactor: Ligand PLP 1 x PLP
5 bound ligands:
Ligand EPE 2 x EPE
Ligand PGE 1 x PGE
Ligand GOL 4 x GOL
Ligand CA 4 x CA
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P43212
Unit cell:
a: 91.49Å b: 91.49Å c: 233.501Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.162 0.252
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Structural and Functional Aspects of Targeting the Secreted Human Group IIA Phospholipase A2.
Kim et al. (2020)
1 more

3 mentions without citation

Detect, correct, retract: How to manage incorrect structural models.
Wlodawer et al. (2018)
2 more