› 5v3j

X-ray diffraction
2.06Å resolution

mouseZFP568-ZnF1-10 in complex with DNA

Released:
DOI: 10.2210/pdb5v3j/pdb
PDB entry 5v3j coloured by chain, front view.
PDB entry 5v3j coloured by chain, side view.
PDB entry 5v3j coloured by chain, top view.
Source organisms:
Primary publication:
DNA Conformation Induces Adaptable Binding by Tandem Zinc Finger Proteins.
Patel A, Yang P, Tinkham M, Pradhan M, Sun MA, Wang Y, Hoang D, Wolf G, Horton JR, Zhang X, Macfarlan T, Cheng X
Cell 173 221-233.e12 (2018)
PMID: 29551271

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119266 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Zinc finger protein 568 Chains: E, F
Molecule details ›
Chains: E, F
Length: 284 amino acids
Theoretical weight: 32.51 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E9PYI1 (Residues: 362-640; Coverage: 42%)
Gene names: Zfp568, Znf568, chato
Sequence domains: Zinc finger, C2H2 type
DNA (26-MER) Chains: A, C
Molecule details ›
Chains: A, C
Length: 26 nucleotides
Theoretical weight: 8.17 KDa
Source organism: Mus
Expression system: Not provided
DNA (26-MER) Chains: B, D
Molecule details ›
Chains: B, D
Length: 26 nucleotides
Theoretical weight: 7.81 KDa
Source organism: Mus
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ZN 20 x ZN
Ligand TRS 2 x TRS
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 55.787Å b: 65.706Å c: 73.022Å
α: 100.52° β: 104.25° γ: 97.25°
R-values:
R R work R free
0.194 0.192 0.237
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Transposable elements shape the evolution of mammalian development.
Senft et al. (2021)
21 other articles

3 mentions without citation

DNA Conformation Induces Adaptable Binding by Tandem Zinc Finger Proteins.
Patel et al. (2018)
2 more