› 5v33

X-ray diffraction
3.49Å resolution

R. sphaeroides photosythetic reaction center mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-ray Transparent Microfluidic Chip

Released:
DOI: 10.2210/pdb5v33/pdb
PDB entry 5v33 coloured by chain, front view.
PDB entry 5v33 coloured by chain, side view.
PDB entry 5v33 coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
X-ray transparent microfluidic chips for high-throughput screening and optimization of in meso membrane protein crystallization.
Schieferstein JM, Pawate AS, Sun C, Wan F, Sheraden PN, Broecker J, Ernst OP, Gennis RB, Kenis PJA
Biomicrofluidics 11 024118 (2017)
PMID: 28469762

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142934 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 240 amino acids
Theoretical weight: 26.02 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 11-250; Coverage: 92%)
Gene name: puhA
Sequence domains:
Structure domains:
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 281 amino acids
Theoretical weight: 31.45 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 302 amino acids
Theoretical weight: 33.87 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-303; Coverage: 98%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like

Ligands and Environments

4 bound ligands:
Ligand BPH 2 x BPH
Ligand BCL 4 x BCL
Ligand FE 1 x FE
Ligand U10 1 x U10
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P42212
Unit cell:
a: 102.332Å b: 102.332Å c: 240.333Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.22 0.262
Expression system: Cereibacter sphaeroides

Quick links

Citations

3 citation in other articles

High-throughput in situ X-ray screening of and data collection from protein crystals at room temperature and under cryogenic conditions.
Broecker et al. (2018)
2 more

1 mention without citation

X-ray transparent microfluidic chips for high-throughput screening and optimization of in meso membrane protein crystallization.
Schieferstein et al. (2017)