› 5uzs

X-ray diffraction
2.37Å resolution

Crystal Structure of Inosine 5'-monophosphate Dehydrogenase from Clostridium perfringens Complexed with IMP and P200

Released:
DOI: 10.2210/pdb5uzs/pdb
PDB entry 5uzs coloured by chain, front view.
PDB entry 5uzs coloured by chain, side view.
PDB entry 5uzs coloured by chain, top view.
Source organism: Clostridium perfringens ATCC 13124
Entry authors: Maltseva N, Kim Y, Mulligan R, Makowska-Grzyska M, Gu M, Gollapalli DR, Hedstrom L, Joachimiak A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101672 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine-5'-monophosphate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 363 amino acids
Theoretical weight: 38.48 KDa
Source organism: Clostridium perfringens ATCC 13124
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2YRZ7 (Residues: 1-88, 216-484; Coverage: 74%)
Gene names: CPF_2558, guaB
Sequence domains: IMP dehydrogenase / GMP reductase domain
Structure domains: Aldolase class I

Ligands and Environments

8 bound ligands:
Ligand IMP 4 x IMP
Ligand 8L4 4 x 8L4
Ligand PGE 5 x PGE
Ligand PEG 6 x PEG
Ligand EDO 2 x EDO
Ligand MG 1 x MG
Ligand K 2 x K
Ligand PG4 1 x PG4
1 modified residue:
Ligand ALY 4 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21212
Unit cell:
a: 111.124Å b: 144.396Å c: 87.527Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.17 0.238
Expression system: Escherichia coli BL21(DE3)

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